Fluorescence detection

© 2015 Masson Group | conception: H. Yockell-Lelièvre

Instrumentation for SPR and Plasmonics

The SPR instrument was modified with a spectrophotometer covering the range of Au NP plasmon resonance to perform LSPR on the same instrument.  The sensors, made of surface-deposited Au NP arrays, present a shorter sensing depth but a higher surface sensitivity. The sensors were validated for the detection of methotrexate and for testosterone.

Nanohole arrays provide a simple platform for sensitive plasmonic sensing, that are amenable in highly multiplexed format. High sensitivity with nanohole arrays can be reached with high incidence angle of the light beam on the nanohole array. A multi-well plate reader was constructed for a standard format 96-well plate and with scanning angle capacity. The multi-well plate reader was validated with protein sensing and for methotrexate.

Portable SPR instrumentation

Microdialysis chamber for whole blood monitoring

Campus MIL (B-5215)
1375 Ave. Thérèse-Lavoie-Roux
Montréal, QC, Canada
H2V 0B3

Automated multi-well plate reader for large arrays of nanoholes

The combination of SPR and fluorescence was realized on the small and portable SPR instrument for dual detection of proteins with SPR and a fluorescent ELISA. We have designed a small motorized fluorescence unit mounted on the SPR system. The fluorescence unit scans each channel sequentially to provide a reading of the fluorescence signal.

A small and portable surface plasmon resonance instrument was designed with three sensing channels and one reference channel. The instrument is powered and controlled with two USB cables. The SPR sensor is based on a dove prism and either a smooth Au mirror or microhole arrays. For more information, please visit the website of Affinité Instruments (affiniteinstruments.com), a start-up company emerging from our laboratory, commercializing our SPR instrument.

The analysis of whole blood is currently challenging with biosensors due to the interference of the components of whole blood. We have designed a microdialysis chamber capable of filtering cells and platelets, in addition to create a diffusion barrier for large biomolecules. We are applying the microdialysis chamber for the detection of small molecules such as therapeutic drugs in whole blood.

LSPR on a Dove prism